Human Lipocalin-2/NGAL Antibody Summary
Gln21-Gly198
Accession # P80188
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1757) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin-2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Lipocalin‑2/NGAL in Human Pancreas. Lipocalin-2/NGAL was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1757) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane of ductal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line, HT-29 human colon adenocarcinoma cell line, and human bone marrow tissue. PVDF membrane was probed with 0.2 µg/mL of Rat Anti-Human Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1757) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin-2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Lipocalin-2/NGAL by Western Blot Silencing of LCN2 impairs cell cycle‐associated proteins. (A) The top proteins downregulated in LCN2‐silenced cells compared with control cells after RPPA proteomic analysis. (B) Gene set enrichment analysis of RPPA data identified pathways that are enriched or downregulated in control vs LCN2‐silenced SUM149 cells. (C) STRING interaction network of predicted active kinases based on enrichment of kinase substrates and protein interactions identified using KEA. The confidence of the interaction is reflected by the edge thickness. Based on node distribution analysis, four central proteins were identified (MAPK1, MAPK8, RPS6KB1, and MTOR). (D) Silencing of LCN2 in SUM149 cells reduced pMEK and pERK expression. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34342930), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Lipocalin-2/NGAL by Western Blot LCN2 was highly expressed in tumors from patients with IBC. (A) High LCN2 expression was associated with shorter overall survival in a meta‐dataset of patients with non‐IBC. (B, C) LCN2 mRNA expression was higher in tumors from IBC patients vs non‐IBC patients in two independent breast cancer datasets [IBC World Consortium Dataset; GSE45582]. (D) LCN2 mRNA expression was higher in ER‐negative compared to ER+ samples IBC samples. (E) LCN2 mRNA expression was higher in more aggressive molecular subtypes, ERBB2+ and TNBC, compared to HR‐positive/HERBB2‐negative subtype. (F) LCN2‐high expression correlates with shorter overall survival in patients with IBC. (G) LCN2 mRNA expression was higher in IBC cell lines compared to non‐IBC cell lines. (H, I) LCN2 protein expression was higher in IBC cell lines compared to non‐IBC cell lines shown by (H) immunoblotting or (I) ELISA for secreted LCN2 in supernatants. Bar graphs indicate mean ± SEM from three independent experiments. graphpad prism software was used to obtain the P values, with Mann–Whitney tests used to compare two categories or one‐way analysis of variance to compare three or more categories. Black lines in each group (B–E, and G) indicate mean ± SD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34342930), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lipocalin-2/NGAL
Members of Lipocalin family share a highly conserved fold with an eight-stranded antiparallel beta barrel, and act as a transporters, carrying small molecules to specific cells (1). Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules (2). It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9) (2). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation (3). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3-5). Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM) (6). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).
- Flower, D.R. et al. (1994) FEBS Lett. 354:7.
- Kjeldsen, L. et al. (1993) J. Biol. Chem. 268:10426.
- Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
- Devireddy, L.R. et al. (2001) Science 293:829.
- Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
- Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
- Flo, T.H. et al. (2004) Nature 432:917.
- Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
Product Datasheets
Citations for Human Lipocalin-2/NGAL Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
22
Citations: Showing 1 - 10
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Subcloning and characterization of highly metastatic cells derived from human esophageal squamous cell carcinoma KYSE150 cells by in vivo selection
Authors: Masafumi Okuda, Jun Inoue, Naoto Fujiwara, Tatsuyuki Kawano, Johji Inazawa
Oncotarget
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Tumour stroma-derived Lipocalin-2 promotes breast cancer metastasis
Authors: B Ören, J Urosevic, C Mertens, J Mora, M Guiu, RR Gomis, A Weigert, T Schmid, S Grein, B Brüne, M Jung
J Pathol, 2016-04-29;0(0):.
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Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy
Authors: Barbara Mara Klinkhammer, Sonja Djudjaj, Uta Kunter, Runolfur Palsson, Vidar Orn Edvardsson, Thorsten Wiech et al.
Journal of the American Society of Nephrology
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The contribution of IL-17A-dependent low LCN2 levels to Helicobacter pylori infection: Insights from clinical and experimental studies
Authors: Cheng, M;Zheng, Y;Fan, Y;Yan, P;Zhao, W;
International immunopharmacology
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Molecular Profiling of Tissue Samples with Chronic Rejection from Patients with Chronic Lung Allograft Dysfunction: A Pilot Study in Cystic Fibrosis Patients
Authors: F Lunardi, DI Abbrescia, L Vedovelli, F Pezzuto, F Fortarezza, GM Comacchio, V Guzzardo, P Ferrigno, M Loy, C Giraudo, AS Fraia, E Faccioli, F Braccioni, E Cozzi, D Gregori, GM Verleden, F Calabrese, FP Schena, F Rea
Biomolecules, 2023-01-03;13(1):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis
Authors: F Yao, Y Deng, Y Zhao, Y Mei, Y Zhang, X Liu, C Martinez, X Su, RR Rosato, H Teng, Q Hang, S Yap, D Chen, Y Wang, MM Chen, M Zhang, H Liang, D Xie, X Chen, H Zhu, JC Chang, MJ You, Y Sun, B Gan, L Ma
Nature Communications, 2021-12-17;12(1):7333.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Nanotherapeutic Modulation of Human Neural Cells and Glioblastoma in Organoids and Monocultures
Authors: I Zhang, P Lépine, C Han, M Lacalle-Au, CX Chen, R Haag, TM Durcan, D Maysinger
Cells, 2020-11-07;9(11):.
Species: Human
Sample Types: Cell Lysates, Organoid, Whole Cells
Applications: ICC, IHC, Western Blot -
Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies
Authors: S An, I Raju, B Surenkhuu, JE Kwon, S Gulati, M Karaman, A Pradeep, S Sinha, C Mun, S Jain
Ocul Surf, 2019-04-06;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Subcloning and characterization of highly metastatic cells derived from human esophageal squamous cell carcinoma KYSE150 cells by in vivo selection
Authors: Masafumi Okuda, Jun Inoue, Naoto Fujiwara, Tatsuyuki Kawano, Johji Inazawa
Oncotarget
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Reactive astrocytes secrete lcn2 to promote neuron death.
Authors: Bi, Fangfang, Huang, Cao, Tong, Jianbin, Qiu, Guang, Huang, Bo, Wu, Qinxue, Li, Fang, Xu, Zuoshang, Bowser, Robert, Xia, Xu-Gang, Zhou, Hongxia
Proc Natl Acad Sci U S A, 2013-02-19;110(10):4069-74.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Lipocalin 2 expression is associated with aggressive features of endometrial cancer.
Authors: Mannelqvist M, Stefansson I, Wik E, Kusonmano K, Raeder M, Oyan A, Kalland K, Moses M, Salvesen H, Akslen L
BMC Cancer, 2012-05-06;12(0):169.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Neutrophil gelatinase-associated lipocalin (NGAL) expression is dependent on the tumor-associated sigma-2 receptor S2RPgrmc1.
Authors: Mir SU, Jin L, Craven RJ
J. Biol. Chem., 2012-03-14;287(18):14494-501.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
Authors: Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG
J. Immunol., 2008-11-15;181(10):7420-7.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Urinary biomarkers predict brain tumor presence and response to therapy.
Authors: Smith ER, Zurakowski D, Saad A, Scott RM, Moses MA
Clin. Cancer Res., 2008-04-15;14(8):2378-86.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Neutrophil gelatinase-associated lipocalin is expressed in osteoarthritis and forms a complex with matrix metalloproteinase 9.
Authors: Gupta K, Shukla M, Cowland JB, Malemud CJ, Haqqi TM
Arthritis Rheum., 2007-10-01;56(10):3326-35.
Species: Human
Sample Types: Synovial Fluid
Applications: Western Blot -
Urinary lipocalin-2 is associated with renal disease activity in human lupus nephritis.
Authors: Pitashny M, Schwartz N, Qing X, Hojaili B, Aranow C, Mackay M, Putterman C
Arthritis Rheum., 2007-06-01;56(6):1894-903.
Species: Human
Sample Types: Urine
Applications: Western Blot -
Pretreatment of carprofen impaired initiation of inflammatory- and overlapping resolution response and promoted cardiorenal syndrome in heart failure
Authors: Veena Krishnan, David Booker, Gabrielle Cunningham, Jeevan Kumar Jadapalli, Vasundhara Kain, Amanda B. Pullen et al.
Life Sciences
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The iron load of lipocalin-2 (LCN-2) defines its pro-tumour function in clear-cell renal cell carcinoma
Authors: C Rehwald, M Schnetz, A Urbschat, C Mertens, JK Meier, R Bauer, P Baer, S Winslow, FC Roos, K Zwicker, A Huard, A Weigert, B Brüne, M Jung
Br. J. Cancer, 2019-11-27;0(0):.
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Neutrophil Gelatinase-Associated Lipocalin and its Receptors in Alzheimer's Disease (AD) Brain Regions: Differential Findings in AD with and without Depression.
Authors: Dekens DW, Naude PJ, Engelborghs S et al.
J Alzheimers Dis
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Heterogeneity and Cancer-Related Features in Lymphangioleiomyomatosis Cells and Tissue
Authors: Roderic Espín, Alexandra Baiges, Eline Blommaert, Carmen Herranz, Antonio Roman, Berta Saez et al.
Molecular Cancer Research
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Lipocalin 2 promotes inflammatory breast cancer tumorigenesis and skin invasion
Authors: Emilly S. Villodre, Xiaoding Hu, Richard Larson, Pascal Finetti, Kristen Gomez, Wintana Balema et al.
Molecular Oncology
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An experimental study of ascorbic acid effects in acute renal failure under general anesthesia
Authors: Omur Ozturk, Sefer Ustebay, Huseyin Avni Eroglu, Murat Günay, Yasemen Adali, İlksen Donmez et al.
Acta Cirurgica Brasileira
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Formalin-fixed lung sections
